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Protein detection using antibodies stored in TBST for over 4 years. A Representative Western blot images using Cyclin D3 and <t>CD71</t> antibodies diluted at 1,000x and stored in TBST for 1,690 and 1,592 days, respectively. B The signal intensities showed a positive correlation with the amount of total lysate ( n = 3)
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Protein detection using antibodies stored in TBST for over 4 years. A Representative Western blot images using Cyclin D3 and CD71 antibodies diluted at 1,000x and stored in TBST for 1,690 and 1,592 days, respectively. B The signal intensities showed a positive correlation with the amount of total lysate ( n = 3)

Journal: Biological Procedures Online

Article Title: Long-term Storage of Diluted Antibody in Tris-buffered Saline with Tween 20

doi: 10.1186/s12575-025-00313-1

Figure Lengend Snippet: Protein detection using antibodies stored in TBST for over 4 years. A Representative Western blot images using Cyclin D3 and CD71 antibodies diluted at 1,000x and stored in TBST for 1,690 and 1,592 days, respectively. B The signal intensities showed a positive correlation with the amount of total lysate ( n = 3)

Article Snippet: The following antibodies were used in this research: α-tubulin (SCBT, sc-5286), β-actin (SCBT, sc-47778), GAPDH (SCBT, sc-32233), cyclin D3 (CST, 2936P), CD71 (CST, 13113 S), CDK4 (CST, 2906), p21 Waf1/Cip1 (CST, 2947), LAMP1 (CST, 9091 S), p53 (SCBT, sc-126), GM130 (SCBT, sc-55591), Histone H3 (Abcam, ab1791), and POLD1 (BD, BD610972).

Techniques: Western Blot